Figure 1
From: Construction and validation of a Bovine Innate Immune Microarray
Effect of DNA element quantity on signal from fluorescently labelled random oligonucleotides. The fluorescence intensities of Panomer™ 9 random oligodeoxynucleotides, Alexa Fluor® 532 conjugate (Molecular Probes, Invitrogen) were measured after hybridisation to β-actin(◆) and GAPDH (■) control elements. These elements were spotted in a gradient of 25, 50, 100, 200 or 400 pg DNA per spot. Error bars represent one standard deviation of the mean.