Figure 2
Variation in amplification-induced fold-change for the conserved and rejected gene-sets. Per gene the fold-change induced by the second round of amplification over all samples was averaged (x-axis) and plotted against the standard deviation (SD) of the fold-change (y-axis). Statistically conserved genes (black) and rejected genes (gray) displayed high variation in standard deviations. A cut-off value at which the 95% normal CI lies between 0.5 and 2 times the expression value (± 0.3 on log10-scale) corresponds to an SD of 0.15 (the 95% normal CI lies within 1.96 standard deviations of the mean; in this case SD = 0.3/1.96 = 0.15).