Table 3 Array quality index. The SD of the log10-intensities in channel 1 give an indication of the dynamic range obtained from the test samples (1000 or 250 cell samples). The correlation coefficients between gene expression log10-ratios of the experiment and log10-intensities of channel 2 (reference sample) were calculated to confirm that the gene expression ratios are not determined by the signal intensities of the reference channel.
From: Limitations of mRNA amplification from small-size cell samples
| Â | Arrays hybridized with sample size 1000 cells | Arrays hybridized with sample size 250 cells | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Array number | 2 | 12 | 1 | 3 | 4 | 11 | mean | 6 | 9 | 7 | 5 | 8 | 10 | mean |
Standard deviation of test channel signal intensity | 0.6 | 0.49 | 0.3 | 0.39 | 0.52 | 0.59 | 0.48 | 0.45 | 0.53 | 0.3 | 0.34 | 0.3 | 0.38 | 0.38 |
Correlation ratio vs. reference channel signal intensities | 0.13 | 0.23 | 0.35 | 0.29 | 0.26 | 0.2 | 0.24 | 0.27 | 0.28 | 0.26 | 0.25 | 0.48 | 0.43 | 0.33 |