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Table 4 Concentration of controls in dilution 1 pools for modified latin square experiments

From: Proposed methods for testing and selecting the ERCC external RNA controls

Pool Concentration Group A (Controls 1–24) Concentration Group B (Clones 25–48) Concentration Group C (Clones 49–72) Concentration Group D (Clones 73–96)
Pool 7 125 1 5 25
Pool 8 25 125 1 5
Pool 9 5 25 125 1
Pool 10 1 5 25 125
  1. Concentration is given as mass ratios, so that "125" represents 1:125,000 or 1 ng of RNA transcript per 125 ng of background RNA where the spike amount is adjusted for its length.