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Table 3 Experimental analysis of 118 genes from microarray hybridisation

From: Large-scale genetic variation of the symbiosis-required megaplasmid pSymA revealed by comparative genomic analysis of Sinorhizobium meliloti natural strains

P-value classes N° of ORFs   Total ORFs amplified by PCR* N° of ORFs positive to PCR amplification N° of ORFs analysed by Southern blotting N° of ORFs with duplication after Southern hybridisation** N° of sequenced ORFs N° of ORFs with nucleotide variation in the 70-mer oligo sequence
p < 0.001 66 log2-ratio < 0 19 19 7 7 - -
   log2-ratio > 0 47 8 - - 8 8
p > 0.001 52 log2-ratio < 0 21 21 4 0 - -
   log2-ratio > 0 31 31 - - 7 0
  1. * PCR amplification was carried out with primer anchored to the flanking regions of the gene.
  2. ** Determined as higher number of observed fragments after Southern-blot analysis of Rm1021 and of the wild strain.