Figure 1

Process pipeline. Observational checks within the pipeline are shaded in grey. Absence of bacterial colonies, no-grows, and unusual observations are recorded on logsheets then entered into the FMA database. A. Verification of the colony picking procedure to ensure that all original clones are accounted for in the source microtiter plates. B. To further confirm A, we stamp a replicate of each microtiter plate containing transformed bacterial cultures onto agar plates. The resulting pattern of colonies is examined to determine presence or loss of DNA in each well of the source microtiter plate. C. Every 384-well culture plate is visually examined for presence of DNA after bacterial DNA culturing. D. Agarose gel electrophoresis is used to evaluate presence and quality of prepared and purified template DNA. E. During sequencing reactions, all volume additions are visually verified and manually adjusted using a single channel pipettor where necessary.