Table 1 Primer sequences for study protocol and its variations.
From: A robust method for the amplification of RNA in the sense orientation
Primer Name | Sequence | Concentration (ng/μL) |
|---|---|---|
T3N9 | 5'-GCGCGAAATTAACCCTCACTAAAGGGAGANNNNNNNNN-3' | 100 |
T7N9 | 5'-GGCCAGTGAATTGTAATACGACTCACTATAGGGAGGCGGNNNNNNNNN-3' | 100 |
Oligo(dT)24 | 5'-TTTTTTTTTTTTTTTTTTTTTTTT-3' | 100 |
T3-Oligo(dT)24 | 5'-GCGCGAAATTAACCCTCACTAAAGGGAGATTTTTTTTTTTTTTTTTTTTTTTT-3' | 100 |
T7-Oligo(dT)24 | 5'-GGCCAGTGAATTGTAATACGACTCACTATAGGGAGGCGGTTTTTTTTTTTTTTTTTTTTTTTT-3' | 100 |
Random Hexamer | 5'-NNNNNN-3' | 3000 |