Figure 1
From: A qualitative assessment of direct-labeled cDNA products prior to microarray analysis
RNA and Cy5-dUTP labeled cDNA measured by the Agilent 2100 Bioanalyzer. Electropherogram images (one representative experiment) of RNAs treated with RNase as follows: No RNase (A, pink), 0.01 ng ml-1 (B, blue), 0.1 ng ml-1 (C, red), 1 ng ml-1 (D, green). Overlayed electropherogram image of Cy5-dUTP labeled cDNA (E, colors as described above, one representative experiment). The first peak (as shown by an arrow) from the left in all electropherograms is a 50 base pair marker present in the sample buffer (M). The second peak (as shown by an arrow) that is present only in Cy5 samples is free Cy5-dUTP (Cy5) and measures approximately 150 nucleotides (data not shown). Though it is a single nucleotide, the Cy5 modification is large, which could explain its delayed migration and detection.