Figure 2

Tissue distribution of mMagT1 mRNA. A, Northern blot analysis of mMagT1 mRNA in MDCT cells or mouse tissues. Tissues were harvested and poly(A)⁺ RNA prepared by standard techniques. Each lane was loaded with 8 µg of poly(A)⁺ RNA. The same blot was stripped and hybridized with ³²P-labeled β-actin as a control for loading. B, real-time reverse transcription PCR analysis of mMagT1 RNA in tissues harvested from mice maintained on normal magnesium diet. mMagT1 and murine β-actin RNA was measured with Real-Time RT PCR (AB7000^TM, Applied Biosystems) using SYBR Green^TM fluorescence. Standard curves for MagT1 and β-actin were generated by serial dilution of each plasmid DNA. The expression level of the mMagT1 transcript was normalized to that of the mouse β-actin transcript measured in the same 1.0 μg RNA sample. Results are normalized to the small intestine and expressed as fold-difference. Mean mRNA levels of kidney, colon, heart, brain, lung, and liver tissues were significantly greater, p>0.01, than small intestine ans spleen.