|
Segment
|
1
|
2
|
3
|
All segments
|
|---|
|
Plate numbers
|
1–288
|
289–576
|
577–816
|
1–816
|
|
Plate count
|
288
|
288
|
240
|
816
|
|
Empty wells
|
1982 (1.79%)
|
2830 (2.56%)
|
2975 (3.23%)
|
7787 (2.49%)
|
|
Non-recombinant clones
|
0
|
5
|
4
|
9
|
|
Non-insert clones 1
|
1.2% 2
|
2.8% 3
|
2.3% 4
|
2.0%
|
|
Recombinant clones 5
|
~107 000
|
~105 000
|
~87 000
|
~299 000
|
|
Average insert size
|
189 Kbp
|
190 Kbp
|
186 Kbp
|
188 Kbp
|
|
Genomic coverage
|
6.8X
|
6.6X
|
5.4X
|
18.8X
|
- Genomic DNA obtained from Salmon sperm was partially digested with EcoRI restriction enzyme and cloned in pTARBAC2.1 vector.
- 1 Estimated numbers for each segment is based upon NotI digestion.
- 2 3 out of 249 clones were identified as non-insert clones
- 3 6 out of 218 clones were identified as non-insert clones
- 4 5 out of 220 clones were identified as non-insert clones
- 5 Recombinant clones have been estimated from the total well number subtracting non-insert clones, non-recombinant clones and empty wells.
