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Table 3 Details of the three segments of the BAC library

From: A highly redundant BAC library of Atlantic salmon (Salmo salar): an important tool for salmon projects

Segment 1 2 3 All segments
Plate numbers 1–288 289–576 577–816 1–816
Plate count 288 288 240 816
Empty wells 1982 (1.79%) 2830 (2.56%) 2975 (3.23%) 7787 (2.49%)
Non-recombinant clones 0 5 4 9
Non-insert clones 1 1.2% 2 2.8% 3 2.3% 4 2.0%
Recombinant clones 5 ~107 000 ~105 000 ~87 000 ~299 000
Average insert size 189 Kbp 190 Kbp 186 Kbp 188 Kbp
Genomic coverage 6.8X 6.6X 5.4X 18.8X
  1. Genomic DNA obtained from Salmon sperm was partially digested with EcoRI restriction enzyme and cloned in pTARBAC2.1 vector.
  2. 1 Estimated numbers for each segment is based upon NotI digestion.
  3. 2 3 out of 249 clones were identified as non-insert clones
  4. 3 6 out of 218 clones were identified as non-insert clones
  5. 4 5 out of 220 clones were identified as non-insert clones
  6. 5 Recombinant clones have been estimated from the total well number subtracting non-insert clones, non-recombinant clones and empty wells.