Hybridization pattern of human (panels A and B) and canine (panels C and D) mRNAs to 2nd and 11th probe pairs of 203133_at Affymetrix probe set. The 203133_at probe set contains 11 probe pairs of which 2nd and 11th represented by anchored oligonucleotide sequences. Corresponding probe pairs identified at the bottom and comprised of perfect match probe (PM) and mismatch probe (MM). The purposely mutated 13th nucleotide in MM sequences is shown as "X". The target mRNAs represented by solid lines aligned to oligonucleotide sequences and mismatched regions depicted as humps. Numbers on the top of each target mRNA represent intensity values generated by MAS 5.0 (Figure 2 A and B). Dotted lines depict nucleotide bond involved in formation of predicted hairpin structure of the 2nd probe of the 203133_at probe set.