Comparison between polyA mRNA and nuclear run-on RNA of immediate early gene activation in Jurkat T cells. A.1 Heatmap of relative gene expression intensities (Z scores). A.2 Graphical representation of the same data illustrating an immediately apparent up-regulation of gene expression in NRO but not polyA mRNA. B. Single end-point PCR validation of up-regulation in polyA mRNA by 1 hour of a subset of genes shown to be activated within 5 minutes by nuclear run-on RNA. C. Patterns of polyA mRNA and nuclear run-on RNA levels for NFKB1 and its inhibitor (NFKBIA).