Variance source
|
Contribution to the variance (%)
|
---|
Gene (G)
|
5.0
|
Dye (SG)
|
4.2
|
Gene × Arrayb
|
7.8
|
RNA isolation and labelingc
|
1.5
|
Sampling
|
7.1
|
VG
d
|
26.9
|
Day × Gene
|
19.7e
|
Experimenter × Gene
|
17.3e
|
Array batch × Gene
|
14.9e
|
Spot pins × Gene
|
4.5f
|
- a The degrees of freedom results in the separate ANOVAs are listed in the supplementary web-site [21].
- b Assumed to consist of hybridization effects and signal-to-noise differences per slide.
- c Derived from the variance observed between Ba and Bb cultures (Figure 1).
- d Variances that are dependent on the validation experiment performed and due to day-to-day differences, identity of the experimenter, and DNA microarray batch differences.
- e Due to overlap in levels, the contribution of these interactions were individually determined.
- f A change from 8 to 12 spot-pins used for array spotting coincided with a switch in the RNA isolation method.