Table 3 Primer Pairs used in this study.
From: Four genomic islands that mark post-1995 pandemic Vibrio parahaemolyticus isolates
Primers | Anneal Temp | Product size | Primer sequences |
|---|---|---|---|
VPaI-1 | Â | Â | Â |
VP0379F-VP0379R | 52.2 | 589nt | Forward 5'-CAGTAATCAAAGTCGGAGC -3' Reverse 5'-ATAACTGCGAGAGGTGC -3' |
VP0380F-VP0380R | 50.7 | 1004nt | Forward 5'-ATCTCTGGGATCAGTCAGG -3' Reverse 5'-GCGATAACGAGTCACTG -3' |
VP0382F-VP0384R | 50.4 | 1809nt | Forward 5'-CGTATTCCATGAATGTCGAG -3' Reverse 5'-GCTCTGCGTAATGCTTC -3' |
VP0387F-VP0387R | 51.9 | 1422nt | Forward 5'-CCTAGATTTCAAAGGAAGTGCG-3' Reverse 5'-GTCACGGATATGTTGTCTTG-3' |
VP0388F-VP0392R | 53.4 | 2256nt | Forward 5'-GTTGGTCTTAATGCCCG -3' Reverse 5'-ACGTACTGTTCAAGCCGAC -3' |
VP0395F-VP0398R | 53.2 | 2393nt | Forward 5'-TGTCCGCCAAAAACAGG -3' Reverse 5'-GCTGAGATCATCGAAAGTTACG -3' |
VP0401F-VP0402R | 51 | 1171nt | Forward 5'-GTCTTCTGTGAATGACTGC -3' Reverse 5'-TTAGCTTCTTCGTCACCCC -3' |
VP0404F-VP0404R | 52.1 | 1397nt | Forward 5'-CATGGGTATCAAGGTAGTAG -3' Reverse 5'-TCTCCATTGAGATTGGC -3' |
379F-404R | 55.1 | 2842nt | Forward 5'-ACCATAACTGCGAGAGGTGC -3' Reverse 5'-TGGTCGTACAACAGACCCAG -3' |
Overlapping PCR Primers | Â | Â | Â |
VP0379R-VP0380R | 50 | 2304nt | Forward 5'-ATAACTGCGAGAGGTGC -3' Reverse 5'-GCGATAACGAGTCACTG -3' |
VP0384R-VP0387R | 51 | 2990nt | Forward 5'-CCGTAAGAGAAGAACGTG-3' Reverse 5'-CGAGTTAATGCAAAACTGGG-3' |
VP0384R-VP0387R | 53 | 2035nt | Forward 5'-ATTGGGGTTGGATGTCGTGG-3' Reverse 5'-CGAACGTGTTCAATACTACG-3' |
VPaI-4 | Â | Â | Â |
VP2130F-VP2130R | 52.3 | 1575nt | Forward 5'-GCTTCGATGTTGATAGGC -3' Reverse 5'-GATGAGAGCAAGTTCTCG -3' |
VP2131F-VP2133R | 51.3 | 1785nt | Forward 5'-CACGTATTTCCACTCCAG -3' Reverse 5'-CCCATACTTCACCATCTTTC -3' |
VP2135F-VP2135R | 50.4 | 1653nt | Forward 5'-GGTAAGAGTGAAGAGGTTCAG-3' Reverse 5'-CCGACTGTAAATCTCATCG-3' |
VP2137F-VP2139R | 50.9 | 2914nt | Forward 5'-TCGTGTGAAATGCGAAC -3' Reverse 5'-CGACGTAGACTTTTAGCC -3' |
VP2143F-VP2143R | 50.5 | 1354nt | Forward 5'-GCAGTCGAACTTGAAGAC -3' Reverse 5'-ATGACCTCTCTGCATCC -3' |
VP2145F-VP2145R | 52 | 727nt | Forward 3'-TATCCACCACCCACAAC-3' Reverse 3'-ATGTGCAAGCTGAGTGG-3' |
2130F- 2145R | 53.2 | 2851nt | Forward 5'-GCTTCGATGTTGATAGGC -3' Reverse 5'-ATGTGCAAGCTGAGTGGGTG -3' |
Overlapping PCR primers | Â | Â | Â |
VP2143F-VP2145R | 49 | 3291nt | Forward 5'-GCAGTCGAACTTGAAGAC -3' Reverse 3'-ATGTGCAAGCTGAGTGG-3' |
VP2132F-VP2135R | 50 | 2387nt | Forward 5'-TCCCTCGTTTCTTGCTC-3' Reverse5'-CTTGTCACAATGATCTCCTG-3' |
VP2135F-VP2137R | 51 | 2900nt | Forward 5'-GCTGGATTAGGACTTGATAC-3' Reverse5'-TACTAACCTCCCATCGG-3' |
VP2140F-VP2142R | 52 | 2562nt | Forward 5'-GGCGTAATTTCTCAGATTCC-3' Reverse 5'-GCTAAACGTGTTGCTGTTG-3' |
VPaI-5 | Â | Â | Â |
VP2900F-VP2900R | 50.3 | 1377nt | Forward 5'-GGGGAACCAGTTATAGG-3' Reverse 5'-ACCGTGAGACGCAGTATCAG -3' |
VP2905F-VP2905R | 50.7 | 2035nt | Forward 5'-CTGATGAGCAGTTTCCG -3' Reverse 5'-TCCATTGTTGTTGCAGG -3' |
VP2908F-VP2908R | 50.6 | 2313nt | Forward 5'-GGCATGAAATCTTGCAG-3' Reverse 5'-CTCACATAGTTACATTGCGG |
2898F-2912R | 59.3 | 2800nt | Forward 5'-CAGCCAAGACCACAAAC-3' Reverse 5'-AATCCCTAGCAGGTTGC-3' |
Overlapping PCR primers | Â | Â | Â |
VP2898F-VP2900R | 52 | 3988nt | Forward 5'-CAGCCAAGACCACAAAC-3' Reverse 5'-ACCGTGAGACGCAGTATCAG -3' |
VP2901F-VP2903R | 52 | 2577nt | Forward 5'-AACGCCAAAGTTGCCTG -3' Reverse 5'-CCGTAAGTAAAGGTTCATCTGC -3' |
VP2903F-VP2905R | 53 | 2330nt | Forward 5'-TCACGTTTGAAGCTCGC -3' Reverse 5'-GTGCCGTATTCTCTTTGTAG -3' |
VP2906F-VP2908R | 51.2 | 2197nt | Forward 5'-GCCCATACAAGTATTCACTC -3' Reverse 5'-TAACCCACAACGATCCG -3' |
VPaI-6 | Â | Â | Â |
VPA1251F-VPA1253R | 51.3 | 1169nt | Forward 5'-TCGTAAACACTGCCAGC -3' Reverse 5'-CTAGCAACGGTGATAGC -3' |
VPA1255F-VPA1255R | 51.1 | 2098nt | Forward 5'-GATGGTTGTGATGAGTTTGC -3' Reverse 5'-TGATGAAGCGATATGCC -3' |
VPA1260F-VPA1261R | 51.5 | 2033nt | Forward 5'-GGCTTTAGGTACTGCTACC -3' Reverse 5'-GGAATCACGTTCTCTGG -3' |
VPA1262F-VPA1262R | 54.1 | 1851nt | Forward 5'-GTGACTTCGAGTTCATTTGC -3' Reverse 5'-GCAACAGTGCATCAATTTGCTC -3' |
VPA1263F-VPA1263R | 53 | 2144nt | Forward 5'-CTACGAAGGTAATCTCGC -3' Reverse 5'-TACCCGCATCAGCTAAC -3' |
VPA1266F-VPA1266R | 52.5 | 1879nt | Forward 5'-ATCCCTTATCGCTCGAC-3' Reverse 5'-CGGATTGTGTTATCTCG-3' |
VPA1270F-VPA1270R | 53.2 | 2225nt | Forward 5'-TGGAACACGCATCCTAC-3' Reverse 5'-ACGACTGAGTTGCATCGAG-3' |
VPA1271F-VPA1271R | 51.9 | 432nt | Forward 5'-CAGCACACTTTTACTGGC -3' Reverse 5'-TGCATCGAACTAACCGC -3' |
1253F-1271R | 53 | 782nt | Forward 5'-ACTTCTGGCTGCGTTTC -3' Reverse 5'-CACTTTTACTGGCTTTCAGC -3' |
Overlapping PCR primers | Â | Â | Â |
VPA1251F-VPA1255R | 48 | 4590nt | Forward 5'-TCGTAAACACTGCCAGC -3' Reverse 5'-TGATGAAGCGATATGCC -3' |
VPA1256F-VPA1259R | 50.7 | 1995nt | Forward 5'-GCCGAGTCAATAAATGTTGCAC -3' Reverse 5'-TTCTAGTTGAACGACGC -3' |
VPA1259F-VPA1261R | 52.2 | 1367nt | Forward 5'-AACGTCAATTTCTGGGG -3' Reverse 5'-GCATCTGAGACAAAGACTAAGG -3' |
VPA1264F-VPA1268R | 53 | 2885nt | Forward 5'-CGAACCTTAAATGGACG -3' Reverse 5'-GACTAATGGCAAGTGGG -3' |
Inverse PCR primers | Â | Â | Â |
VP0401F-VP0380R | 49 | 4596nt | Forward 5'-GTCTTCTGTGAATGACTGC -3' Reverse 5'-GCGATAACGAGTCACTG -3' |
VP2143F-VP2133R | 51 | 4614nt | Forward 5'-GCAGTCGAACTTGAAGAC -3' Reverse 5'-CCCATACTTCACCATCTTTC -3' |
VP2910F- VP2900R | 56 | 2939nt | Forward 5'-CAACATGTACCCAGAGTACG -3' Reverse 5'-ACCGTGAGACGCAGTATCAG -3' |
VPA1270F-VPA1255R | 48 | 4462nt | Forward 5'-TGGAACACGCATCCTAC-3' Reverse 5'-GCTGGTGACCTCGATAC-3' |
Genotypic primer pairs | Â | Â | Â |
GS-VP.1 F-GS-VP.2 R | 45 | 651nt | Forward 5'-TAATGAGGTAGAAACA-3' Reverse 5'-ACGTAACGGGCCTACA-3' |
VP0325F-VP0325R | 54 | 901nt | Forward 5'-TGAAAGTAGCCGTTATTGG -3' Reverse 5'-CCATTTAGCGTTTCTAGCATTC-3' |