Skip to main content

Table 2 Number of gene-specific clones in the nurse shark BAC library Single copy genes.

From: Construction of a nurse shark (Ginglymostoma cirratum) bacterial artificial chromosome (BAC) library and a preliminary genome survey

Single copy genes

 

Number of positive clones

%

 

Genes

Library Screening

Colony hybridization

Colony hyb./Library scr.

References

TAP1

60

37

62%

[17]

Ring3

72

62

86%

Unpublished

MHC class I

20

10

50%

[17]

CD83

6

3

50%

[23]

Gici-11*

28

19

68%

[37]

LMP2

18

NA

NA

[17]

TCRβ

9

8

89%

Unpublished

TCRδ

11

10

91%

Unpublished

Possibly two genes in tandem or close vicinity

Genes

Library screening

Colony hybridization

Colony hyb./Library scr.

References

MHC class IIα

14

12

86%

[39]

MHC class IIβ

13

9

69%

[40]

Factor B

16

14

87%

[24]

Multiple genes

Genes

Library screening

Colony hybridization

Colony hyb./Library scr.

References

LMP7**

41

19

46%

[41]

  1. NA: not available
  2. *several genes, but our 3'UTR specific probe only identifies one gene
  3. **only one complete and functional gene (i.e. many pseudogenes)