Nucleotide sequence analyses of the MRP 1 gene in four populations suggest negative selection on its coding region

Wang, Zihua; Sew, Pui-Hoon; Ambrose, Helen; Ryan, Stephen; Chong, Samuel S; Lee, Edmund JD; Lee, Caroline GL

doi:10.1186/1471-2164-7-111

Table 1 Primers and PCR conditions for amplifying and sequencing MRP 1

From: Nucleotide sequence analyses of the MRP 1 gene in four populations suggest negative selection on its coding region

Exons	Primer Direction	Length (bp)	Primer Sequence (5' – 3')	Amplicon Length(bp)	Additional Primer For Sequencing	Tm
Exon 1	Forward	22	GCATTTGAAAAGTGGTCGCAGG	688		59
	Reverse	20	TCCGCAGGAACTGAGTCACC
Exon 2	Forward	20	GCAGAAGACACCACATACCT	510		60
	Reverse	20	AGAAGAAGGAACTTAGGGTC
Exon 3	Forward	19	GCATGGTGACCAGACAAAC	501		60
	Reverse	19	CTCCAGCTGATCATTGCCT
Exon 4	Forward	21	ACGTGGTCCATTAAGAAATAG	571		61
	Reverse	20	GACTTCTACACAAGCCAGAG
Exon 5	Forward	19	CCCAGCCCCAGAATGTGAT	358		58
	Reverse	18	CCCCAGCCACATCTAAGC
Exon 6	Forward	22	TGTTGTATTGTGGTTGCACATG	383		60
	Reverse	20	GAGCTGAGCATGTTCATTCG
Exon 7	Forward	20	TCCCTAAGTCTTTTGTATGC	574	AAGCCATTTTTCCTGCATGAC (forward)	61
	Reverse	19	TACCCCATTTGGCAGAAAA
Exon 8	Forward	20	AGAGAGCTTAAGGACCTTGT	591		59
	Reverse	19	TATGAGCCCACTTCAGGAC
Exon 9	Forward	19	CGTGTTCCCTATGCAATTC	602		59
	Reverse	18	CCTGCCACCTAAGGTCAC
Exon 10	Forward	18	TCCTGGGCAGACAGATAG	439		61
	Reverse	18	TGAACCACAGCCGGAACT
Exon 11	Forward	19	GCTTGGGAGAAAGGAGCGT	452		61
	Reverse	19	TGAGTCCAACTGGCAGGCA
Exon 12	Forward	20	TAATAGACGGTGAAGTTGAG	743	ATGAAGAGCAAAGACAATCG (forward)	61
	Reverse	20	AAGTAATTCTCTTGCCTCAG
Exon 13	Forward	21	GTCGTTGATTTATCCAGTTCA	523		61
	Reverse	20	CTTTCTTTCAGGCATGACCA
Exon 14	Forward	20	TCTGAAATACCTTTTGTGGG	627		60
	Reverse	19	GGTCAAAGCCTTGGAAAGT
Exon 15	Forward	21	TTACAAGGACAAAGCTGCTTG	475		60
	Reverse	19	TGTATCTGCACCCATTGTC
Exon 16	Forward	20	GTTTAGTACAGTCTTGCCTT	463		60
	Reverse	19	CCAAAATCCTGCCTTCTAG
Exon 17	Forward	21	GTGGGCCAGCTGTTGTCTCGT	441		61
	Reverse	20	AGTGAGACCTGAGCCACACC
Exon 18	Forward	18	CGTATTGTGAGTCTCAAG	596	TACCCATTACCACAACTG (reverse)	61
	Reverse	18	TTTTCCGACCCCTCTACC
Exon 19	Forward	18	GAGTTTTGCCCACCAGGT	462		61
	Reverse	19	GGTGGTTTTCCACATTGCT
Exon 20	Forward	18	TATGCCCTTTCCCTCATG	758		61
	Reverse	18	GAGCAAAGACCCACACCA
Exon 21	Forward	19	CTGGAGAGTGACATGGTGG	504		61
	Reverse	20	GGGTGACCCTGAGTAAGTCA
Exon 22	Forward	20	GTTGAATAGCTAAAGGGGAG	599		61
	Reverse	19	TGTAAAATGGGCACACTGG
Exon 23	Forward	20	ATGCCTGGTTCATCATTATT	514		58
	Reverse	20	CTTTAGGTAACACTGGTATA
Exon 24	Forward	20	AGCGTGAGCTATTATTGTCA	727		61
	Reverse	19	ACTCAATAGCAGAGTCGGT
Exon 25, 26	Forward	19	CCCATTGTGCATGTTTTGA	1442	AGATGCAGCTAAAGCAGTTC* GTGACTGATGGGGTTTCG**	61
	Reverse	20	AAAGACTGGACAAGCGTTAA
Exon 27	Forward	22	ACCTACTATGTGCTCTGAGCCC	693		61
	Reverse	20	TACTGCCACAGTCCACTCCC
Exon 28	Forward	18	CGAGTCATTCCTTTTGGG	493		61
	Reverse	20	GTAGGGCCAAATGTATGTTT
Exon 29	Forward	18	AAGGAGCTCTGATACCCC	405		61
	Reverse	19	GGACAAATGGTCATCTGGG
Exon 30	Forward	19	CTCCCAAAGTGCTCAGATT	706		61
	Reverse	19	GAAATGCTTGAACCCCAGA
Exon 31	Forward	21	TCTGAATGTAATGGAACAGTG	1677		61
	Reverse	21	GGAAGAGCATCAGTAACTAAA

*reverse primer for Exon 25, **forward primer for Exon 26

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ISSN: 1471-2164

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