Figure 1

Scheme of the FMDV genome and repertoire of mAb SD6-escape mutants included in the microarray. Top: C-S8c1 genome (8115 nucleotides, excluding homopolymeric tracts); boxes indicate encoded proteins and lines indicate regulatory regions (not drawn to scale); the filled circle represents protein VPg covalently linked to the 5' end of the RNA, and AAAA represents the 3'-terminal polyadenylate tract. Below the genome, the amino acid sequence of the G-H loop of the VP1 protein (amino acids 133 to 156) is shown; the epitope defined by mAb SD6 is underlined. The sequence RGDL involved in integrin recognition is boxed. Below the mAb SD6 epitope sequence, the amino acid replacements found in individual biological FMDV clones isolated as mAb SD6-escape mutants whose corresponding mutations have been analysed in the microarray, are indicated. The discontinuous line at the bottom indicates a double mutant. Based in [11, 13, 44] and references therein.