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Figure 1 | BMC Genomics

Figure 1

From: Colour variation in red grapevines (Vitis vinifera L.): genomic organisation, expression of flavonoid 3'-hydroxylase, flavonoid 3',5'-hydroxylase genes and related metabolite profiling of red cyanidin-/blue delphinidin-based anthocyanins in berry skin

Figure 1

Grapevine flavonoid hydroxylase gene fragments isolated from 'Cabernet Sauvignon' genomic DNA. The position of the conserved sites among plant flavonoid hydroxylases, on which the degenerate primers were designed, and the region of the gene fragments sequenced in grapevine are referenced to the gene Ht1 of Petunia × hybrida [GenBank:AF155332] for the flavonoid 3'-hydroxylase (a) and to the Petunia × hybrida GenBank:Z22544 for the flavonoid 3',5'-hydroxylase (b) [41]. The consensus amino acid sequences of grapevine VvF3'H and VvF3'5'H gene fragments are shown along with the position of the intron and (line below the sequence) the variable sites among all the sequenced fragments. The symbol '-' stands for invariable position, '+' underlines two functional domains invariantly found in plant flavonoid hydroxylases, '*' indicates the site of the 23-bp deletion in the VvF3'H-2 sequence.

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