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Table 2 Primers pairs and PCR conditions used for the amplification of grapevine flavonoid hydroxylase genes.

From: Colour variation in red grapevines (Vitis vinifera L.): genomic organisation, expression of flavonoid 3'-hydroxylase, flavonoid 3',5'-hydroxylase genes and related metabolite profiling of red cyanidin-/blue delphinidin-based anthocyanins in berry skin

Code Sense primer Anti-sense primer Expected size (bp) Annealing temp. (°C)
F3'H F3'H_S F3'H_AS 825* 60
F3'5'H F3'5'H_S F3'5'H_AS 1020* 56
F3 VvF3'H_S VvF3'H_AS 196 58
F3-1 VvF3'H-indel_S VvF3'H_AS 166 58
F35-1 Vv3'5'H-1A_S VvF3'5'H-1A_AS 156 58
F35-2 Vv3'5'H-2A_S Vv3'5'H-2A_AS 152 65
F3int VvF3'H-intron_S VvF3'H-intron_AS 255 58
F35-1int Vv3'5'H-1A-intron_S Vv3'5'H-intron_AS 589 58
F35-2int Vv3'5'H-2A-intron_S Vv3'5'H-intron_AS 593 58
38C24-FM 38C24-FM_S 38C24-FM_AS 312 58
38C24-RM 38C24-RM_S 38C24-RM_AS 330 58
17K14-FM 17K4-FM_S 17K4-FM_AS 327 58
9C17-FM 9C17-FM_S 9C17-FM_AS 358 58
40A22-FM 40A22-FM_S 40A22-FM_AS 327 58
5A23-FM 5A23-FM_S 5A23-FM_AS 218 54
  1. * in absence of introns.