Colour variation in red grapevines (Vitis vinifera L.): genomic organisation, expression of flavonoid 3'-hydroxylase, flavonoid 3',5'-hydroxylase genes and related metabolite profiling of red cyanidin-/blue delphinidin-based anthocyanins in berry skin

Table 2 Primers pairs and PCR conditions used for the amplification of grapevine flavonoid hydroxylase genes.

Code	Sense primer	Anti-sense primer	Expected size (bp)	Annealing temp. (°C)
F3'H	F3'H_S	F3'H_AS	825*	60
F3'5'H	F3'5'H_S	F3'5'H_AS	1020*	56
F3	VvF3'H_S	VvF3'H_AS	196	58
F3-1	VvF3'H-indel_S	VvF3'H_AS	166	58
F35-1	Vv3'5'H-1A_S	VvF3'5'H-1A_AS	156	58
F35-2	Vv3'5'H-2A_S	Vv3'5'H-2A_AS	152	65
F3int	VvF3'H-intron_S	VvF3'H-intron_AS	255	58
F35-1int	Vv3'5'H-1A-intron_S	Vv3'5'H-intron_AS	589	58
F35-2int	Vv3'5'H-2A-intron_S	Vv3'5'H-intron_AS	593	58
38C24-FM	38C24-FM_S	38C24-FM_AS	312	58
38C24-RM	38C24-RM_S	38C24-RM_AS	330	58
17K14-FM	17K4-FM_S	17K4-FM_AS	327	58
9C17-FM	9C17-FM_S	9C17-FM_AS	358	58
40A22-FM	40A22-FM_S	40A22-FM_AS	327	58
5A23-FM	5A23-FM_S	5A23-FM_AS	218	54

ISSN: 1471-2164