Figure 2

Porcine BAC contig with anchoring on porcine RH map and on human sequence after BAC end sequence analysis. The full contig is presented including mainly INRA BAC. Some additional BAC where recruited on the international analysis available at http://www.sanger.ac.uk/cgi-bin/Projects/S_scrofa. The black circles represent sequenced BAC ends (BES) used for screening INRA BAC library. The white circles are BES for which a marker has been developed for a quality control of the contig by PCR on overlapping BAC (positive results are indicated with gray circles). The black boxes represent genes and microsatellites used for screening INRA BAC library, the white boxes indicate additional BAC on which a positive result has been obtained by PCR with these markers. Markers in bold on the RH map belong to the framework map, whereas markers in italic are additional markers mapped at the most likely location (when two locations are as likely, the marker is indicated on the left side of the map and its possible locations are indicated with a line). The BES for which a significant homology has been identified with sequence of HSA6 are illustrated by dotted lines. The large arrows represent the extent and the orientation of the different fragment conserved between pig and human genomes. The small arrows in fragment 5 represent the duplication identified on the human. Finally, the white box in fragment 6 is the fragment absent on the pig genome.