Figure 1

Contig alignment depth and SNP identification. Of the 4,251 UniGenes (contigs) identified, 2,994 were singletons (not shown) and all others had two or more ESTs (i.e., alignment depth of two or greater). The total number of contigs for each alignment depth class is represented by the height of the columns, and the different colors represent different SNP number classes. It should be noted that alignment depth here refers to the number of ESTs in each contig and does not necessarily imply a constant alignment depth at all sites along the contig sequence.