Skip to main content

Table 3 Successful annotation based on BLAST against different gene collections

From: A wing expressed sequence tag resource for Bicyclus anynana butterflies, an evo-devo model

   BLAST hits
Field min E-valuea Totalb Bestc Uniqued
Dmel.pro1 1e-171 1346 110.5 4
Dmel.nuc1 1e-147 264 7 6
Bmori.pro2 0e+00 1730 141.5 194
Bmori nuc2 0e+00 1265 1222 20
Bmori.wgs.nuc2 0e+00 1370 283 143
lep.nuc3 0e+00 1012 459.5 147
invert.pro3 1e-171 1488 170.5 4
NonRed.pro4 1e-175 1487 29 3
SwissP.pro4 1e-176 1136 25 1
organel.nuc5 3e-70 26 3 2
Rfam.nuc5 0e+00 27 2 0
plant.pro 1e-125 793 7.5 0
Ecoli.nuc 4e-12 7 1 1
  1. a The threshold maximum E-value was set to 1e-05. b Total number of contigs with significant E-value. c Number of contigs having the lowest E-value for each specified BLAST field. When the lowest and second lowest E-values were the same for a particular contig it counted as 0.5 for each of the hit fields. d Number of contigs having significant BLAST hits exclusively for one of the fields. Some BLAST fields were combined to give the counts in Figure 2: 1 Dmel, 2 Bmori, 3 InvLep, 4 protein databases, 5 non-nuclear genes (as explained in the Methods). All collections used in our BLAST analysis were downloaded from public databases (see Methods) in June-August of 2005 (except for the organellar nucleotidic and the E. coli whole-genome sequences, both obtained in April 2004).