Figure 2

SMRT-array results showing the deletion on chromosomes 9 and 22 that are the result of the derivative 9 chromosome (der(9)) deletion in a chronic myeloid leukemia (CML) patient. The dotted red line denotes the 2 standard deviation cut-off to identify a deletion while the green dashed line marks the 2 standard deviation cut-off for a gain. A) Chromosome 9 alterations identified using the SMRT-array. The red arrow shows the deletion extending from 9q33.3 to 9q34.1. B) Chromosome 22. The deletion of chromosome 22 material, located around 22q11.2, on the der(9) chromosome is shown by the red arrow. C) Chromosome 14 from the same CML sample with the der(9) deletion, and shown to have the CNV14q12 event using another microarray comparative genomic hybridization platform as well as real-time polymerase chain reaction. The black arrow marks the site of CNV14q12 and, due to the use of a different normal genomic DNA sample, does not display the expected gain of genomic material. The most distal copy number variation was seen only in this sample and therefore not studied further.