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Figure 2 | BMC Genomics

Figure 2

From: An alternative method to amplify RNA without loss of signal conservation for expression analysis with a proteinase DNA microarray in the ArrayTube® format

Figure 2

Comparison of PCR vs. hybridization pattern on the arrays. A. Electrophoresis of PCR products. Initially reverse transcribed c-DNA of human fibroblasts, DLD-1 and 97TM1 was used as template for PCR with specific primers for Matriptase, PAI-1 and PAI-2. Positive controls: plasmid-cDNAs of the three targets, negative controls were template free. Product sizes: matriptase 470 bp, PAI-1 687 bp, PAI-2 710 bp. B. Array analysis. Sample preparation was performed according to the protocol for overall linear amplification with the SPA primer and simultaneous labelling (see Methods part).

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BMC Genomics

ISSN: 1471-2164

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