Table 1 Primers used for standard RT-PCR

TRPC1

NM_011643

371 bp [11]

for: CAAGAT TTTGGGAAATTTCTGG

rev: TTTATCCTCATGATTTGCTAT

TRPC2

NM_011644

326 bp [29]

for: GATCCGGTTCATGTTCATCCT

rev: GAGCGAGCAAACTTCCACTC

TRPC3

NM_019510

317 bp [11]

for: TGACTTCCGTTGTGCTCAAATATG

rev: CCTTCTGAAGCCTTCTCCTTCTGC

TRPC4

NM_016984

414 bp [11]

for: TCTGCAGATATCTCTGGGAAGGATGC

rev: AAGCTTTGTTCGAGCAAATTTCCATTC

TRPC5

NM_009428

339 bp [11]

for: ATCTACTGCCTAGTACTACTGGCT

rev: CAGCATGATCGGCAATGAGCTG

TRPC6

NM_013838

326 bp [11]

for: AAAGATATCTTCAAATTCATGGTC

rev: CACGTCCGCATCATCCTCAATTTC

TRPC7

NM_012035

692 bp

for: CGTGCTGTATGGGGTTTATAATG

rev: GCTTTGGAATGCTGTTAGAC

TRPV1

NM_001001445

320 bp

for: GCATCTTCTACTTCAACTTCTTCGTC

rev: CCACATACTCCTTGCGATGGC

TRPV2

NM_011706

427 bp

for: CGGCACTTCCTCTCT

rev: GTCGGTCACGGTCAA

TRPV3

NM_145099

298 bp

for: CAAGGACTGCCACCACCATC

rev: CATCACAGTTGCCAGAGAGG

TRPV4

NM_022017

249 bp

for: GAGTCCTCAGTAGTGCCTGG

rev: CAACAAGAAGGAGAGCAGTC

TRPV5

NM_001007572

172 bp [30]

for: CGTTGGTTCTTACGGGTTGAA

rev: GTTTGGAGAACCACAGAGCCTCTA

TRPV6

NM_022413

318 bp

for: AACCAGCCTTCCACC

rev: CCTCCATTAGCACCA

TRPM1

NM_018752

446 bp

for: TATCCTATGACACCAAGCCA

rev: GGTCTTCCTTATTCTCCACG

TRPM2

NM_138301

215 bp

for: CAGATCCCAACCTACATTGACG

rev: GAAGGTGTAGTTGAACATGGCGA

TRPM3

NM_177341

497 bp

for: CCTGTTCTTCTGGCA

rev: GCTTCTCTGGCTCCT

TRPM4

NM_175130

176 bp

for: CCCTGAGGATGGTGTTGAGT

rev: AGGAGCACTGGGATGTCAAT

TRPM5

NM_020277

360 bp

for: CAGATACTGAGGATGGCTGG

rev: GGATCTTGGTGGATGTGCTA

TRPM6

NM_153417

219 bp

for: CCAGGTGCCGGTAATAACA

rev: CTCTTGTGGCTGCCTTAGGT

TRPM7

NM_021450

295 bp

for: CGGAGCTGGTCGCACAATTA

rev: CCTGGAAGACATCTGTGAGG

TRPM8

NM_134252

344 bp

for: TCCACGAGACTTCTTCACTT

rev: GTGTATCTGAGGTCAGTCTT

GAPDH

NM_008084

451 bp

for: ACCACAGTCCATGCCATCAC

rev: TCCACCACCCTGTTGCTGTA