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Table 4 In situ localization of TRPC3, TRPC5 and TRPC6 transcripts in different mouse organs

From: Tissue-specific expression of TRP channel genes in the mouse and its variation in three different mouse strains

tissue

TRPC3

TRPC5

TRPC6

Skeletal muscle

   

   • sarcolemma

+

neg.

±

   • sarcoplasmic reticulum

neg.

neg.

neg.

   • myofibrils

neg.

neg.

neg.

Heart

   

   • cardiac muscle cells

+

neg.

±

   • blood vessel endothelium

neg.

neg.

neg.

Aorta

   

   • Tunica muscularis

+

+

n. d.

   • endothelium

+

neg.

n. d.

small intestine

   

   • smooth muscle layer

neg.

±

neg.

   • submucosa

+

±

±

   • enterocytes of the villi

++

+

++

   • enterocytes of the crypts

+++

+++

++

Kidney

   

   • glomeruli

±

neg.

neg.

   • distal tubule

+++

±

++

   • proximal tubule

neg.

neg.

neg.

Lung

   

   • bronchial epithel

+++

+

++

   • pneumocytes

+

±

+

   • blood vessel endothelium

+

neg.

+

Liver

   

   • hepatocytes

+++

++

++

   • epithel of the Ductus interlobularis bilifer

+++

++

++

Spleen

   

   • red pulp

++

neg.

neg.

   • white pulp

neg.

++

++

Pancreas

   

   • exocrine acini

+++

+++

+++

   • islet of Langerhan's

neg.

neg.

neg.

   • duct system

++

neg.

neg.

   • blood vessel endothelium

neg.

neg.

neg.

testis

   

   • leydig cells

+

+

++

   • sertoli cells

neg.

neg.

neg.

   • spermatogonia/spermatocytes

neg.

neg.

+

   • spermatozoa

neg.

neg.

++

Epididymis

   

   • epithelium of the Ductuli efferentes testis

+++

++

++

   • epithelium of the Ductus epididymidis

+

++

++

   • spermatozoa

neg.

neg.

neg.

Uterus

   

   • endometrium – glandular epithelial cells

++

+++

+++

   • myometrium

neg.

++

+

fallopian tube

   

   • smooth muscle layer

neg.

±

neg.

   • tubal epithelium

+

+

+

Ovary

   

   • cortex

   

- granulosa cells

++

+

++

- theca cells

neg.

±

+

- corpus luteum

neg.

±

+

   • medulla

neg.

neg.

+

CNS

   

   • spinal ganglia

+++

+

n. d.

   • cortex

++

±

+

   • hippocampus

+

neg.

+

   • ventricle ependym

+++

±

++

   • basal ganglia

++

neg.

++

   • cerebellum

   

- Purkinje cells

++

±

+++

- Stratum granulosum

+

neg.

+

  1. The in situ hybridization technique was applied to tissue sections as shown in Fig. 3. The obtained signals were classified into six categories: negative (neg.), very weak (±), weak (+), moderate (++), strong (+++) or not determined (n. d.).