Figure 2
From: Expression of target and reference genes in Daphnia magna exposed to ibuprofen
Relative gene expression of target gene Ltb4dh in Daphnia magna following a 24 h exposure to ibuprofen. Mean relative expression (n = 3) ± SD of target gene Ltb4dh (amplification efficiency E = 1.060 ± 0.042) without normalisation (white bars) or with normalisation (black bars) to normalisation factor NF3 based on the geometric mean of Act, GAPDH and UBC (gene name abbreviations as Table 1). Expression levels are displayed relative to the mean control level. An asterisk (*) indicates a significant difference (p < 0.05) between the highest treatment and any other treatment.