Table 1 Candidate reference genes and target gene investigated
From: Expression of target and reference genes in Daphnia magna exposed to ibuprofen
Gene name | Symbol | GenBank accession no. | wFleaBase EST no. | Gene Ontologya | Forward primer (5'-3') | Reverse primer (5'-3') | Amplicon size (bp) |
|---|---|---|---|---|---|---|---|
Actin | Act | AJ292554 | Structural constituent of cytoskeleton (F) GO:0005200 | CCA CAC TGT CCC CAT TTA TGA A | CGC GAC CAG CCA AAT CC | 71 | |
Alpha-tubulin | Atb | WFes0007807 | Microtubule (C) GO:0005874 | TGG AGG TGG TGA CGA CT | CCA AGT CGA CAA AGA CAG CA | 89 | |
Cyclophilin | Cyp | WFes0012034 | Protein folding (P) GO:0006457 | GAC TTT CCA CCA GTG CCA TT | AAC TTT CCA TCG CAT CAT CC | 78 | |
Glyceraldehyde-3- phosphate dehydrogenase | GAPDH | AJ292555 | Glycolysis (P) GO:0006096 | GGC AAG CTA GTT GTC AAT GG | TAT TCA GCT CCA GCA GTT CC | 89 | |
Succinate dehydrogenase | SDH | WFes0009191 | Tricarboxylic acid cycle (P) GO:0006099 | TGC CAT TTA GTC GCA CTC AG | GTG AGC TTG TCC TCC TTT GC | 89 | |
TATA-box binding protein | TBP | WFes0002485 | Transcription initiation (P) GO:0006367 | GCA GGG AAG TTT AGT TTC TGG A | TGG TAT GCA CAG GAG CAA AG | 88 | |
Tryptophanyl- tRNA synthetase | WARS | WFes0003968 | Tryptophanyl-tRNA ligase activity (F) GO:0004830 | GCA CTG TGA TCA GCT CTT GC | TTT CGA TTG GAG GAA ATT CG | 82 | |
Ubiquitin Conjugating Enzyme | UBC | WFes0004602 | Ubiquitin-dependent protein catabolism (P) GO:0006511 | TCA CCT GCA CTC ACC ATT TC | AAT CTC CGG AAC CAA AGG AT | 90 | |
18S ribosomal RNA | 18S | AF070104 | Protein biosynthesis (P) GO:0006412 | CGC TCT GAA TCA AGG GTG TT | TGT CCG ACC GTG AAG AGA GT | 77 | |
28S ribosomal RNA | 28S | AF532883 | Protein biosynthesis (P) GO:0006412 | GAG GCG CAA TGA AAG TGA AG | TGT TCG AGA CGG GAT CA | 70 | |
Leukotriene B4 12-hydroxy- dehydrogenase b | Ltb4dh | DY242190 | Response to toxin (P) GO:0009636 | AAC CTA CAC CGA GGG TTT CG | TCC AAC ATT AAC GCC ATT AAG C | 70 |