Table 2 Primers used for 3'-RACE and RT-PCR
From: Identification and analysis of YELLOW protein family genes in the silkworm, Bombyx mori
Primer name | Primers for 3'-RACE | Primers for RT-PCR |
|---|---|---|
Bm-yellow-d | ATCGCTGTTGACAAAGAAACCCTGCA (1) CACGTCCAGTTTTCAACGAGTAATGAC (2) | ATCGCTGTTGACAAAGAAAC (F) GGCGCTGTGTCATTTCTGTAG (R) |
Bm-yellow-fa | GAGCGACGGAATATTCAGTCTGACTA (1) TTCCATCCTTTGATCAGCACGCACGA (2) | TTCCATCCTTTGATCAGCACGCACGA (F) TCTAGCATAACCGAAACGCGGCA (R) |
Bm-yellow-c | CTGGTCTTGCTGATATTTGGGGTG (1) GAGGACATGAAAGAGGATTCATTCT (2) | ACCTCGAGAGAACGGATTCCGAA (F) CGACCAGGATCCACATTAAGCCT (R) |
Bm-yellow-fb | GGATCCGAAGGTATGGGTCAATG (1) CCTACATAACGGACTTGGCGACTA (2) | CCTACATAACGGACTTGGCGACTA (F) AATACACTACTCGGTCCGTGG (R) |
Bm-yellow-b | CGATAACGCCATCGGTAACTGCA (1) GATTCTCGATAACGGACGAGTGG (2) | AGGTATTGCATCGATCGCTG (F) GACTAAGCAAGCAGGTGTGCT (R) |
Bm-yellow-f2 | GGATATCCCAGGTATAAGGAAGC (1) CCGCATTTCAGGAAAGACATCGA (2) | GGATATCCCAGGTATAAGGAAGC (F) GTAATCTGCGCGATTTTGCGCT (R) |
Bm-yellow | TCACCTCTGAGCAGTCACACAGAA (1) TCGACCCGTATCTTGAGAGACGAA (2) | TCGACCCGTATCTTGAGAGACGAA (F) TGTGCCTGAAGCACTGGCAATG (R) |
Nup and Actin | Nup: AAGCAGTGGTATCAACGCAGAGT | Actin-F: CCCCATCGAACACGGAATCG Actin-R: CGCTCGGCAGTGGTAGTGAA |