Figure 3

Confirmation of SAGE data. (A). Real-time RT-PCR analysis. The relative fold change of UN(+)/UN(-) is plotted. PCR analyses were performed on cDNA from UN(-) or UN(+) tissues. All values represent the mean ± SEM. *p < 0.05 vs control. (B). Western blot analysis of ODC, TCTP, IGFBP7 and Gal-3 proteins in UN(-) and UN(+) tissues. Fifty micrograms of total cell protein were loaded per lane on a 10% SDS-polyacrylamide gel, and ODC, TCTP, IGFBP7 and Gal-3 were detected by immunoblotting with antibodies against the respective proteins. As a control for proteins loading in SDS-PAGE, an anti-β-actin antibody was used.