Table 3 Results of the protein-level approach (TreeSAAP) showing the amino acid sites and physicochemical amino acid properties influenced by positive-destabilizing selection among eutherian mammal ABAD/HSD10 proteins.
Amino acid site | Properties | Amino acid site | Properties |
|---|---|---|---|
Substrate binding-cleft sites | 56 | Pα, α m , pH i | |
95 | Pα, pK' | 57 | N s , B r, μ |
98 | B l , R F , α n , R a , H p , pK', H, N s , B r , μ | 59 | K0, M w , V0, M w , H t |
202 | Pβ, B l , K0, R a | 62 | K0, C a , M w , V0 |
214 | pH i , F, P, α c , P t | 64 | α c |
Sites in insertion 1 region of loop D | 70 | α c | |
102 | N s , B r , E l , H p , E t | 73 | P α |
103 | B l , C a , H, Mw, V0, F | 76 | K 0 |
106 | P c , pK', F, P t | 80 | Pα, α m |
108 | Pα, E sm | 119 | N s , Pβ, B r , H p , E t |
Other sites | 123 | α n , R a , H p | |
5 | C, R a | 135 | P r , P, α m |
7 | P c , pK', F, P t | 177 | N s , Pβ, B l , R F , R a , H p , H t |
15 | α c | 189 | α n , R a , H p , α c |
25 | Pα, E sm | 194 | α c |
28 | α m | 237 | K0, M w , V0 |
44 | N s , B r , H p , E t , Pβ | 239 | E sm , Pα |
46 | pK', F, P r , α c | 240 | α n |
49 | E sm , Pα | 244 | C a , M w , V0 |
50 | F | ||