Figure 2

Evolutionary tree of UCP gene family inferred using the neighbor-joining algorithm. Genetic distance proportional to branch length was calculated based on amino acid difference (p-distance) with complete deletion of gaps. Bootstrap values computed after 1,000 random replications are indicated at tree nodes. Accession numbers for UCPs are: [GenBank: AAH69556, NM_003355, AAC18822, AF110532, AF078544] for human (Homo sapiens) UCP1, UCP2, UCP3, UCP4 and UCP5, respectively; [GenBank: NM_009463, AAD17198, NM_009464, NM_028711, NM_011398] for mouse (Mus musculus) UCP1, UCP2, UCP3, UCP4 and UCP5, respectively; [GenBank: NM_012682, AB005143, AB006614] for rat (Rattus norvegicus) UCP1, UCP2 and UCP3, respectively; [GenBank: NM_001003046, AB020887, NM_001003047] for Dog (Canis familiaris) UCP1, UCP2 and UCP3, respectively; [GenBank: NM_131176, BI474135] for Zebrafish (Danio rerio) UCP2 and UCP5, respectively; [GenBank: AAS10175, AJ243486] for Carp (Cyprinus carpio) UCP1 and UCP2, respectively; [GenBank: NM_214049, AAD08811] for pig (Sus scrofa) UCP2 and UCP3, respectively; [GenBank NM_133018.2, AAK92857] for Drosophila UCP4 and UCP5, respectively; and [GenBank: AAH44682, NM_133018] for frog (Xenopus laevis) UCP2 and UCP4, respectively.