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Table 2 Characterization of transposon mutants with an auxotrophic phenotype

From: Random mutagenesis in Corynebacterium glutamicum ATCC 13032 using an IS6100-based transposon vector identified the last unknown gene in the histidine biosynthesis pathway

Number of clones

Auxotrophic requirement

Integrated in locus a

Integrated in gene a

Encoded protein

Functional complex

Reference

Single auxotrophy

      

8

Adenine

cg3063 cg2876

purA (5) purB (3)

Adenylosuccinate synthase Adenylosuccinate lyase

AMP biosynthesis

[38]e

6

Guanine

cg0703 cg0699 cg1216

guaA (2) guaB2 (2) nadA (1)

putative GMP synthase, Inositol-monophosphate dehydrogenase, Quinolate synthase

GMP biosynthesis Quinolinate biosynthesis

[37]d [91]e

8

Uracil

cg1816 cg0617 (1)

pyrB (1) -

Aspartate carbamoyltransferase catalytic chain, Putative Molybdopterin-guanine dinucleotide biosynthesis protein

Pyridmidine biosynthesis

[92]e

2

Hypoxanthine

cg2857 b

purF (1) b

Amidophosphoribosyltransferase

Purine biosynthesis

[93]f

5

Arginine

cg1586

argG (1)

Argininosuccinate synthase

Arginine biosynthesis

[94]

6

Phenylalanine

cg2391 cg3207

aroG (2) pheA (4)

Phospho-2-dehydro-3deoxyheptonate aldolase, Prephenate dehydratase

Phenylalanine biosynthesis

[95]

[96]

16

Histidine

cg2299, cg2303, cg2305, cg1699, cg2297, cg1698, cg2296, cg0910

hisA (1), hisB (4),hisD (3), hisE (1), hisF (2), hisG (2), hisI (1), hisN (1)

Phosphoribosylformimin-5-aminoimidazole carboxamide ribotideisomerase, Imidazoleglycerol-phosphate dehydratase, Histidinol dehydrogenase, Anthranilate synthase component I, probabale Cyclase (imidazole glycerol phosphate synthase-subunit), Phosphoribosyltransferase, prob. Phosphoribosyl-AMP cyclohydrolase, Histidinol-phosphate phosphatase

Histidine biosynthesis

[97]

[49]

  

cg2237

thiO (1)

Putative D-amino acid oxidase flavoprotein oxidoreductase

Thiamine biosynthesis

[42]g

11

Proline

cg0490

proC (1)

Pyrroline-5-carboxylate reductase

Proline biosynthesis

[98]

5

Methionine

nd c

nd c

   

13

Serine

cg1451

serA (1)

Phosphoglycerate dehydrogenase

Serine biosynthesis

[99]

17

Cysteine

cg3118

cysI (2)

Ferredoxin-sulfite reductase

Assimilatory sulfate reduction

[65]

14

Leucine

cg1488

leuD (1)

3-Isopropylmalate dehydratase, small chain

Leucine biosynthesis

[100]

6

Threonine

cg1338

thrB (1)

Homoserine kinase

Threonine biosynthesis

[101]

2

Lysine

cg1334

lysA (2)

Diaminopimelate decarboxylase

Lysine biosynthesis

[102]

46

Tryptophan

cg3364, cg3363, cg3362, cg3361, cg3359, cg3360

trpA (5), trpB (11), trpCF (10), trpD (9),trpE (6), trpG (2)

Tryptophan synthase alpha chain, Tryptophan synthase beta chain, Indole-3-glycerol-phosphate synthase/phosphoribosylanthranilate isomerase, Anthranilate phosphoribosyltransferase, Anthranilate synthase component I, Anthranilate synthase component II

Tryptophan biosynthesis

[43]

1

Biotin h

cg2147 (1)

 

BioY family membrane protein

Biotin transporter

 

1

Pyridoxine

cg0897

pdxR (1)

Pyridoxine biosynthesis transcriptional regulator, Aminotransferase

Pyridoxine/valine biosynthesis

[47]

Double auxotrophy

      

8

Arginine/Uracil

cg1813 cg1814

carB (1) carA (1)

Carbamoyl phosphate synthase subunit, Carbamoyl phosphate synthase small subunit

Arginine/pyrimidine biosynthesis

[103]e

4

Valine/Isoleucine

cg1435 cg1437

ilvB (2) ilvC (2)

Acetolactate synthase, Acetohydroxy acid isomeroreductase

Isoleucine/valine biosynthesis

[40]

Alternative requirement

      

1

Arginine or Cytosine

nd c

    

1

Cysteine or Pyridoxine

cg0156 (1)

 

ROK-type transcriptional regulator

Cysteine/pyridoxine biosynthesis

 

1

Proline or Threonine

cg1238 (1)

 

putative membrane protein

  

3

Methionine or Cobalamin

cg1290

metE (2)

Homocysteine methyltransferase

Methionine biosynthesis

[41]

  1. a Numbers in brackets indicate the number of clones allocated to the respective gene.
  2. b Integration 30 nt upstream of start codon
  3. c nd, not determined.
  4. d Referenced for Corynebacterium ammoniagenes.
  5. e Referenced for Escherichia coli.
  6. f Referenced for Mycobacterium smegmatis
  7. g Referenced for Rhizobium etli.
  8. h Supplementable with 10-fold amounts of biotin compared to the wild-type strain
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BMC Genomics

ISSN: 1471-2164

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