Figure 2

KIR3DL0 protein sequences in primates. The multiple sequence alignment includes human (hs), common chimpanzee (pt), gorilla (gg), rhesus monkey (mm) and common marmoset (cj). The suffixes 'c' and 'g' denote cDNA-derived or predicted from genomic sequence, respectively. Sequences are split according to their exonic boundaries. The frameshift causing deletion of seven bases in IgD2 of the human gene has been manually corrected by insertion of three Xs (boxed) to bring the sequence back into frame. The human cDNA sequence is shown in italics after the frameshift as it is not part of the multiple sequence alignment. Dots (.) indicate identity with human KIR3DL0g and dashes (-) indicate insertions/deletions of amino acids. Inversely labeled amino acids designate polymorphic positions where one allele is identical to hsKIR3DL0g and another as indicated. Inhibitory motifs (ITIM) are highlighted in grey. Asterisks (*) indicate cysteine residues that are likely to form disulfide bridges. N-linked glycosylation signals (NXS/T) are shown by hashes (#) when conserved between the majority of primate sequences and plus signs (+) when present in only marmoset. Putative beta-bulges containing the WSXS/PS or L/VSAPS motifs are delineated by double bars (=) while single bars (-) mark partially conserved motifs. Two adjacent copies of KIR3DL0 are present in marmoset with one of them (cjKIR3DL0g2) missing the last three exons.