Figure 2
Ratio/intensity (m/a) plot deduced from DNA microarray hybridizations comparing the transcriptome of the dtxR mutant C. glutamicum IB2103 with that of the wild-type strain C. glutamicum ATCC 13032. The analyzed strains were cultivated in low-iron CGXII medium to mid-exponential growth phase. Then, 10 mg/l FeSO4 was added to the medium, and the cultures were incubated for further 15 min. Subsequently, total RNA was isolated from two biological replicates and used for DNA microarray hybridizations including label swapping. Genes showing enhanced expression in the dtxR mutant C. glutamicum IB2103 are marked by black diamonds; genes with decreased expression are indicated by black triangles; genes without differential expression pattern are shown by gray spots. Differentially expressed genes later on assigned to the DtxR regulon of C. glutamicum are named by their identifiers. Genes were regarded as being differentially expressed with a-values equal or greater than 7.0 and m-values equal or greater than 1 (up-regulation) or equal or smaller than -1 (down-regulation).