Figure 5

Functional dissection of genes belonging to the DtxR regulon of C. glutamicum. Genes and gene clusters showing differential expression in the dtxR mutant C. glutamicum IB2103 are presented. The respective values of differential gene expression shown above the arrows were deduced from DNA microarray hybridizations. Genes with decreased expression in C. glutamicum IB2103 are shown as white arrows; genes without a given value were detected only by real-time RT-PCR (Table 1). Predicted Rho-independent transcriptional terminators are indicated by hairpin structures, DtxR binding sites by white boxes. The DtxR binding site in front of cg0466 and cg3119 might also be involved in regulation of expression of genes on the opposite DNA strand [14]. The identified genes were grouped into seven functional classes (A-G) according to proposed physiological functions of the encoded proteins.