Figure 2

Changes in gene expression in human mast cells following IgE sensitization and FcεRI aggregation. Changes in expression over control of human cord blood derived mast cells that were activated by IgE sensitization and FcεRI crosslinking for different time points (2 hr, 6 hr and 12 hr). Labelled cRNA from cell of each time point were hybridized to Human Genome Focus array and signals were scanned after fluidics. The data was analyzed as described in material and methods and analysis revealed differential expression of 760 genes between resting and stimulated mast cells with statistical significance (P ≤ 0.05). Agglomerative average-linkage hierarchical clustering of the five different experimental conditions was obtained for selected groups of genes using GeneSpring 7.0. Each colored box represents the normalized expression level of a given gene in a particular experimental condition and is colored according to the color bar. The data represent average of four independent experiments.