Figure 4

Real-Time PCR of selected genes. Comparison of gene expression from control/resting mast cells (Con); after mast cells were sensitized by human IgE (Sen), and following a time course of FcεRI aggregation for 2 hours (2 h), 6 hours (6 h) and 12 hours (12 h). 1 μg of total RNA was used from the same sample which has been used for microarray experiments at different time points for Light-Cycler Real-Time PCR. The concentrations of these transcripts were calculated using respective standard curves. (A) IL-1β expression; (B) IL-6 expression; (C) IL-8 expression; (D) MCP3 expression; (E) RANTES expression; and (F) SPHK1 expression. The data represent average ± the standard deviation of four different samples.