Table 2 Sequences of PtMKKs " 3'RACE and RTqPCR primers used for 3'UTR MAPKK gene isolation and expression profiling.
Group | Gene name | 3' RACE Primers (5' to 3') | Forward RTqPCR Primers (5' to 3') | Reverse RTqPCR Primers (5' to 3') |
|---|---|---|---|---|
A | PtMKK2-1 | TGGTATTGCTGGAGTGTGCAACAGGCCA | TGCAAATGCATCAAACTCCTG | CCACCCAAAAGAGACATCAG |
PtMKK2-2 | CTCCACCTTCTGCACCACCAGACCAATT | AGCTTGCAAACTATGAGGAAC | CGATTCTGTCAGGGAAAACC | |
PtMKK6 | GCTGCAACCCTCCAGCTCAGGTCCC | GGGAAGGTTGTCATCTTTGG | TGGGTAATTCACAGGAGGTTC | |
B | PtMKK3 | GCAGTTGCAATCCGCGTTTCAGGATCC | GCTCTATGTTCCTCCAGTTAC | GAAATGGTTTCATATCTGTCACG |
C | PtMKK4 | ATTGCTTGTTGTTTGCAGAGGGAGCCGG | GAATTGTTGTGGGTTATTGTTG | GGTCAGAACTAAAGGTGCTGTG |
PtMKK5 | CCCAATAACCGCATCACTGTCGATGAGG | AGCATCCGTTTATTGTGAGAAG | AACTACCTACTAAGCAATTGGC | |
D | PtMKK7 | CGAGCTTGCCGGAGGGAGCATCTGAGGA | TGACCCGAATGCCCAGTTAA | TTCCTCTCATTTCAAGAGCAG |
PtMKK9 | GCGTCGGAGGAGTTTCGGGACTTTATTC | ACTTTATTCAGTGTTGCCTGC | GGGTCAAATCACAAGTCCAC | |
PtMKK10 | TGTGGAGAGAAACCAGACTGGGCAGCAT | TTTGGTCTGGATATAAGCTGTG | TGCAAAACCAGCATCATCTTC | |
PtMKK11-1 | GGGAGTGCTCTGCAGTTGTTGCAACACC | TGCAACACCCTTTTATACTGC | TGATGGAAGTGACAGGATGG | |
PtMKK11-2 | GGAGAATCCCCTAGCTTCCCAAAGGAAG | CCTATGTTTGCTTAGGGGAG | TTGGTCCAAGAGCCATTTCG |