Time course analysis of expression of the histidine and serine biosynthesis genes after treatment with DL-serine hydroxamate. The samples were taken in five minute intervals up to a total of 25 minutes. The relative amount of mRNA was measured by real-time RT-PCR with a LightCycler instrument. The expression ratios were determined by calculating the ratio of t0 (untreated) and t5–25 (treated) samples from four experiments. Below the diagrams, the organization of the respective genes in the C. glutamicum chromosome is depicted.