Evaluation of marker gene expression stability during chemostat cultures with Trihoderma reesei RutC30. A. Dry weight (blue squares), off gas CO2 concentration (black line), protein concentration (g g-1 DW) (red diamonds) and specific cellobiohydrolase activity (nkat g-1 DW) (orange circles) and ammonium concentration (green triangles) in chemostat cultures I – III (D = 0.03 h-1). In culture III arrow indicates the addition of trace mineral solution. B. Average fold-change of expression from predicted steady state of 30 marker genes (F
). Error bars indicate the average deviation in Fss between the different marker genes. C. Expression ratios of genes showing the highest variation between batch and continuous phase (increasing expression, red symbols cbh1, egl1) and (decreasing expression, green symbols gpd1, hsp70). Residence time 0 indicates the start of the continuous growth phase. Expression levels as log2 expression ratios from the F
predicted steady state expression level.