Expression profile of mRNAs from L. major syntaxins. Fragments obtained after semi-nested RT-PCR reactions on cDNA obtained from total RNA extracted from L. major promastigotes (A) or amastigotes (B). The fragment sizes are indicated on the right (in kb); -RT denotes that the reverse-transcriptase was not included in the initial reaction. The 5' primer used was specific for the "splice-leader" sequence, whereas the 3' primers were specific of each individual members of the Qa group. The star denotes an artefactual product.