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Table 1 Primer and target statistics

From: Design factors that influence PCR amplification success of cross-species primers among 1147 mammalian primer pairs

Parameter valuea
number of primer pairs 1147
number with no IS-mismatches 955
spacing in human genome 2.3 +/- 2.4 Mb (0.0 – 23.2)
product sizeb 1130 +/- 514 bp (197 – 3862)
primer length 21.8 +/- 3.1 b (16 – 38)
annealing temperature 61.0 +/- 1.4°C (55.0 – 69.0)
introns crossed 1.08 +/- 0.36 (0 – 4)
  1. aFor values that vary, data are given as average +/- standard deviation or range.
  2. bThese values are based upon the human target.
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