Probe level relationships between STAT binding and mRNA transcription in response to IFN. Differences in probe intensities for chromosome 22 arrays are plotted for the responses of HeLaS3 cells stimulated with IFN-γ or IFN-α (relative to non-stimulated controls). ΔSTAT = differential STAT binding; ΔE = differential expression. (A) IFN-γ (ΔE) vs IFN-γ (ΔSTAT1). (B) IFN-α (ΔE) vs IFN-α (ΔSTAT1). (C) IFN-α (ΔE) vs IFN-α (ΔSTAT2). (D) IFN-γ (ΔE) vs IFN-α (ΔSTAT2). (E) IFN-γ (ΔSTAT1) vs IFN-α (ΔSTAT1). (F) IFN-γ (ΔSTAT1) vs IFN-α (ΔSTAT2). All raw data were obtained from Hartman et al.'s recent analysis of transcription and STAT binding in response to IFN . All values are on the log2 scale. Red lines indicate cutoffs of ± 0.5 (on the log2 scale).