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Table 2 Oligonucleotide primers. Oligonucleotide primers used in PCR assays to clone different segments of the toxin-antitoxin system from pGI1.

From: TasA-tasB, a new putative toxin-antitoxin (TA) system from Bacillus thuringiensis pGI1 plasmid is a widely distributed composite mazE-doc TA system

Segment Region amplified (Accession number : AY138809)a Oligonucleotide primersb Primer names Length (bp) of amplified region
A (304–629)C 5'-TTAGGCATATG ACAGCAAACACTCGCCACATGG-3' DOTF 345 bp
   5'-AATATGTCGAC TCATTTCGGTTTTCCTACTTTCCTCC-3' DOTR2  
P (8147–8254,1–332)C 5'-GGCCCGGTACC GTGGAGGAAAGTAGGAAAACCG-3' pOIf 459 bp
   5'-CCCGTCGAC CCCACGTCTTTTTTTAATTTAAAGG-3' pOIr  
AP (8147–8254,1–629)C 5'-TTAGGCATATG ACAGCAAACACTCGCCACATGG-3' DOTF 754 bp
   5'-CCCGTCGAC CCCACGTCTTTTTTTAATTTAAAGG-3' pOIr  
pAP (8147–8254,1–861)C 5'-AACGGTTAATACGTTCTAACTG-3' ValC 978 bp
   5'-CCCGTCGAC CCCACGTCTTTTTTTAATTTAAAGG-3' pOIr  
p2AP (8147–8254,1–939)C 5'-GGCTCTAGAATCTTCCCTATC-3' ValC2 1056 bp
   5'-CCCGTCGAC CCCACGTCTTTTTTTAATTTAAAGG-3' pOIr  
RTA (370...630)C 5'-AATGACAGCAAACACTCGCC-3' RTAf 261 bp
   5'-GCACCGTCAAATTTATTGATGG-3' RTAr  
RTP (8249–8254,1–228)C 5'-GAGGTGTTAAAGATACAGG-3' RTPf 234 bp
   5'-AATCTGAAGCTTCTTGTTCGG-3' RTPr  
RTAP (8249–8254,1–630)C 5'-AATGACAGCAAACACTCGCC-3' RTAf 636 bp
   5'-AATCTGAAGCTTCTTGTTCGG-3' RTPr  
  1. a: C indicates complementary sequences.
  2. b: Letters in bold refer to restriction sites.