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Table 1 List of TILLING targets, sizes of amplicons and number and type of mutations identified for each gene.

From: TILLING is an effective reverse genetics technique for Caenorhabditis elegans

Gene Name Description Gene Size (bp) PCR Size (bp) 1Prev. alleles 2Prev. strains 3Mis- sense 3Null 3Silent 3Total
*C05C10.5 Hypothetical protein 788 1175 0 0 2   1 3
mel-32 C05D11.11 Serine hydroxyl-methyl-transferase 1600 1500 16 1 4 1 2 7
mus-81 C43E11.2 Endonuclease MUS81 2530 1171 1 0 4   3 7
xpf-1 C47D12.8 Structure-specific endonuclease ERCC1-XPF 9112 1452 0 0 5   3 8
*F25H2.13 Helicase of the DEAD superfamily 4985 1499 1 0 5   4 9
htp-3 F57C9.5 HIM-3 paralogue 2598 1452 1 1 5   5 10
*M03C11.2 Helicase of the DEAD superfamily 5943 1490 1 0 4   1 5
cki-2 T05A6.2 Hypothetical protein 1555 1569 0 0 7 1 2 10
mdf-2 Y69A2AR.30 Spindle assembly checkpoint protein 4461 1466 1 0 1   5 6
htp-2 Y73B6BL.2 HIM-3 paralogue protein 2 1199 1451 0 0 5   1 6
Totals    14225    42 2 27 71
  1. * Gene name not assigned
  2. 1 Number of mutant alleles listed in Wormbase [7] as existing prior to this study.
  3. 2 Number of mutant strains available from the Caenorhabditis Genetic Stock Center.
  4. 3 Number of mutations of this type identified in this TILLING study.
  5. Missense mutations alter the amino acid sequence of the encoded protein. Null mutations refer to mutations that convert an amino acid codon into a premature stop codon, or that alter a conserved splice junction and result in premature truncation of the protein product of the gene. Silent mutations are changes that do not affect the protein product of the gene. These include mutations in introns or intergenic sequences, and mutations that alter the third bp of a codon in such a way that it does not change the amino acid encoded by that codon.