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Figure 1 | BMC Genomics

Figure 1

From: Novel design and controls for focused DNA microarrays: applications in quality assurance/control and normalization for the Health Canada ToxArray™

Figure 1

Schematic of the design of one sub-grid of the ToxArray™. Each sub-grid is printed in quadruplicate by one pin. Printing proceeds left-to-right across rows and top row to bottom row. Spot A (bottom row) represents a buffer-only negative control that is printed immediately following another buffer-only spot. B1 through B3 spots are 100 μM A. thaliana spots which reach saturation. Spots C1 through C3 are buffer-only spots that are printed immediately following B spots. The average signals from these spots were used to calculate the amount of cross-spot contamination (relative to Spot A) using the formula: [ ( C ¯ A ) / ( B ¯ A ) ] × 100 MathType@MTEF@5@5@+=feaafiart1ev1aaatCvAUfKttLearuWrP9MDH5MBPbIqV92AaeXatLxBI9gBaebbnrfifHhDYfgasaacH8akY=wiFfYdH8Gipec8Eeeu0xXdbba9frFj0=OqFfea0dXdd9vqai=hGuQ8kuc9pgc9s8qqaq=dirpe0xb9q8qiLsFr0=vr0=vr0dc8meaabaqaciaacaGaaeqabaqabeGadaaakeaacqGGBbWwcqGGOaakcuqGdbWqgaqeaiabgkHiTiabbgeabjabcMcaPiabc+caViabcIcaOiqbbkeaczaaraGaeyOeI0IaeeyqaeKaeiykaKIaeiyxa0Laey41aqRaeGymaeJaeGimaaJaeGimaadaaa@3E8D@

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