Table 1 Primers used in this study
Primer | Nucleotide sequence (5'-3') | Relevant characteristics |
|---|---|---|
orf4-1 | 5'-CGCGGATCCCATTCATTTTTGAATATTTATG-3' | forward primer, to construct pBMB165-F4A |
orf5-1 | 5'-CGCGGATCCCTTCATGGCCAAGTAACTTAA-3' | forward primer, to construct pBMB165-F5C, pBMB165-F1B |
orf5-2 | 5'-GGCGAGCTCCATGAGTGGGAAGCAGATATT-3' | reverse primer, to construct pBMB165-F5C, pBMB165-ORIB |
orf5-3 | 5'-CGCGGATCCCAATTGAAAATTAACCAGCTG-3' | forward primer, to construct pBMB165-ORIB |
orf5-4 | 5'-TCCCCCGGGATTTTCAATTGTCCACTGTGC-3' | reverse primer, to construct pBMB165-F1B |
orf6-1 | 5'-AGCAGTTACGTAAATGGAATACAAGATG-3' | forward primer, to construct pBMB165-F6A, pBMB165-F6B, pBMB165-F6D, pBMB165-F6E |
orf6-2 | 5'-CGAGAGCTCTACACATTTACACATTTACAC-3' | reverse primer, to construct pBMB165-F6A, pBMB165-F4A |
orf6-3 | 5'-CGAGAGCTCACTTGTTGTAACGTTATAGAC-3' | reverse primer, to construct pBMB165-F6B |
orf6-4 | 5'-CGAGAGCTCATTTATCTCTATACTGTGTTC-3' | reverse primer, to construct pBMB165-F6D, pBMB165-F6G |
orf6-5 | 5'-CGAGAGCTCATAATCTTAGTTGTTCAGGTG-3' | reverse primer, to construct pBMB165-F6E |
orf6-6 | 5'-CTGGAATTCTCCACGTGTTGGCTTAAATAC-3' | forward primer, to construct pBMB165-F6G |