Coverage of four BAC clones with sequence contigs assembled from sequence reads produced by 454 sequencing technology. a. Relationship between coverage and number of sequence contigs from two independent sequencing experiments 1 (blue) and 2 (red) for all four BACs. Because the BACs have different sizes, the number of contigs is normalised. b. Numbers of sequence contigs in different size ranges from experiment 1. Assembly of 454 sequences resulted for all four BAC clones in a few large and many small sequence contigs. c. Percentage of the total size of the BACs covered by sequence contigs of different size ranges from experiment 1. The cumulative size of all contigs was in all four cases smaller than the actual size of the BAC clone (percentage in parentheses underneath the BAC name). This is due to pooling of repetitive sequences into consensus contigs. For BAC 604D5 and 509D2, the percentage was calculated based on size estimates from agarose gels.