Densitometric analysis of Western blot experiments and microarrays of β-catenin, EGFR, c-myc, cytokeratin 19 protein and mRNA. Proteins and mRNAs were extracted from undifferentiated control cell culture grown in collagen I and medium, or differentiated cultures which were differentiated either by TGFβ1 or soluble factors secreted by IMR-90 cells. Results from the expression ratios from differentiated cell cultures were compared to undifferentiated control culture. The calculated values are from four β-catenin, five cytokeratin 19 two EGFR and two c-myc labeled Western immmunoblots. The EGFR, β-catenin, cytokeratin 1 and c-myc results were from three separate microarrays, EGFR having two separate probes on the filter. Bars indicate the standard deviation of separate experiments (Fig. 1A). Two representative Western blots subjected to densitometric analysis (Fig. 1B).